Method for staining and preserving agar gel diffusion plates.

In situ cytoenzymatic identification of human bone marrow colonies grown in agar: a simple method with automated cytochemistry reagents.

Human bone marrow culture in agar is a widely used technique for the study of haemopoietic progenitor cells in both normal and pathological states.' The colonies formed are heterogeneous, consisting of neutrophil, monocyte, eosinophil, and mixed neutrophil monocyte colonies. Methods for differentiating colony types have been described,1'8 but they are cumbersome, require removal of the agar fro...

Comparison of ELISA and Agar Gel Diffusion Techniques in Diagnosis of Human Hydatidosis.

Agarose overlays allow simplified staining of polyacrylamide gels.

Laser scanners from Molecular Dynamics (Sunnyvale, CA, USA) or Hitachi Scientific Instruments (Mountain View, CA, USA) are being used increasingly to detect DNA in polyacrylamide gels. The standard technique for visualizing DNA is to separate the plates of a gel and pour a diluted nucleic acid stain such as ethidium bromide or SYBR Green I [from Molecular Probes (Eugene, OR, USA); also markete...

Direct and Transdentinal (Indirect) Antibacterial Activity of Commercially Available Dental Gel Formulations against Streptococcus mutans

OBJECTIVE To evaluate the direct and transdentinal (indirect) agar diffusion antibacterial activity of different commercially available antibacterial dental gel formulations against Streptococcus mutans. MATERIALS AND METHODS The commercially available dental gel formulations were Corsodyl® (COG, 1% chlorhexidine), Cervitec® (CEG, 0.2% chlorhexidine + 0.2% sodium fluoride), Forever Bright® (F...

Agar diffusion method for the differentiation of Bacillus anthracis.

A method was developed for identification of Bacillus anthracis based on elaboration of protective antigen by individual colonies and its detection by double-diffusion precipitation in agar plates.